Anti-COMMD5 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9GZQ3

Core Product Specifications and Parameters

Biological Background: COMMD5 Function and Localization

• COMMD5 (COMM domain-containing protein 5), also known as HCaRG (hypertension-related calcium-regulated gene protein), is a member of the COMMD protein family.
• It functions as a scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex includes the CCC subcomplex and the retriever subcomplex. Related references: PMID:37172566, PMID:38459129
• COMMD5 may modulate the activity of cullin-RING E3 ubiquitin ligase (CRL) complexes. Related references: PMID:21778237
• It negatively regulates cell proliferation and cell cycle G2/M phase transition, likely by transactivating p21/CDKN1A through a p53/TP53-independent signaling pathway.
• COMMD5 is involved in kidney proximal tubule morphogenesis.
• It down-regulates activation of NF-kappa-B. Related references: PMID:15799966
• Subcellular localization: Predominantly found in the cytoplasm and nucleus.
• Tissue specificity: Highly expressed in heart, stomach, jejunum, kidney, liver, and adrenal gland, with generally higher expression in adult organs than in fetal tissues.

Experimental Guidance and Technical Tips

• The immunogen covers full-length human COMMD5 (aa 1-224); consider verifying cross-reactivity with other species if needed, as the sequence is highly conserved.
• For Western blotting, an expected band around 25 kDa should be validated in relevant sample lysates; include appropriate controls.
• In immunocytochemistry/immunofluorescence, assess subcellular localization; COMMD5 can appear in both cytoplasm and nucleus depending on cell type and conditions.
• For ELISA, optimize antigen coating and antibody dilution using purified recombinant COMMD5 or peptide standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For COMMD5-related research in cancer, kidney development, and NF-kappa-B signaling, our team can assist in sourcing rare-target raw materials and technical services to accelerate your assay development from concept to clinic.