Anti-COMMD1 Polyclonal Antibody for IF/ICC, ELISA - Q8N668

Core Product Specifications and Parameters

Biological Background: COMMD1 Function and Localization

• COMMD1 is a scaffold protein in the commander complex, essential for endosomal recycling of transmembrane cargos, comprising CCC and retriever subcomplexes. Related references: PMID:37172566 PMID:38459129
• It down-regulates NF-kappa-B activity by promoting ubiquitination and proteasomal degradation of the RELA subunit. Related references: PMID:15799966 PMID:17183367 PMID:20048074
• Plays a role in copper ion homeostasis by binding one copper ion per monomer and facilitating ATP7A trafficking between the trans-Golgi network and vesicles. Related references: PMID:17309234 PMID:25355947
• Modulates membrane expression and ubiquitination of ion channels including ENaC, SLC12A2, and CFTR, thereby regulating Na+ and Cl- transport. Related references: PMID:14645214 PMID:20237237 PMID:21741370 PMID:23515529 PMID:21483833
• Competes with ARNT/Hif-1-beta for binding to HIF1A, resulting in impaired transcriptional activation by HIF-1. Related references: PMID:20458141
• Negatively regulates neuroblastoma G1/S cell cycle progression and proliferation by stimulating ubiquitination of RELA and NF-kappa-B degradation. Related references: PMID:28604741
• Subcellularly localizes to the nucleus, cytoplasm, and endosome-associated membranes including early and recycling endosomes.
• Ubiquitously expressed with highest levels in the liver; frequently down-regulated in cancer tissues.

Experimental Guidance and Technical Tips

• For IF/ICC, since COMMD1 localizes to multiple compartments, consider using appropriate fixation and permeabilization protocols to preserve antigenicity. Titrate antibody to minimize background.
• Validate antibody specificity in cells with known knockdown or knockout of COMMD1, or compare with isotype controls.
• For ELISA, the antibody may be used to detect recombinant or endogenous COMMD1; include appropriate positive and negative controls. Determine optimal coating and detection conditions.
• Since the immunogen corresponds to the N-terminal region (aa 1-190), consider potential isoform specificity; verify in target species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, serving diagnostic manufacturers and research labs from concept to clinic. This anti-COMMD1 polyclonal antibody supports research into endosomal recycling, NF-kB signaling, and copper homeostasis — and we can supply validated antibody pairs, optimized polyclonal or monoclonal antibodies, and bulk ancillary reagents for your assay development. Whether you need rare target raw-material sourcing or technical consulting, CamelBio streamlines your procurement with reliable, high-quality products.