Anti-Collagen XVII/COL17A1 Rabbit mAb for WB, IHC-P, ELISA - Q9UMD9

Core Product Specifications and Parameters

Biological Background: Collagen XVII/COL17A1 Function and Localization

• Collagen XVII, also known as 180 kDa bullous pemphigoid antigen 2 (BP180) and bullous pemphigoid antigen 2, is encoded by the COL17A1 gene and belongs to the collagen superfamily, featuring a transmembrane domain and multiple collagenous repeats.
• It is a key structural component of hemidesmosomes, playing a critical role in the attachment of basal keratinocytes to the underlying basement membrane, thereby contributing to dermal-epidermal cohesion.
• The protein undergoes proteolytic processing to generate a 120 kDa linear IgA disease antigen (LAD-1), which serves as an anchoring filament component and is targeted by autoantibodies in linear IgA bullous dermatosis.
• COL17A1 is primarily localized to cell junctions, specifically hemidesmosomes, and is also found as a secreted protein in the extracellular matrix and basement membrane.
• Tissue expression is predominantly in stratified squamous epithelia, including skin, cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra, and thymus; it is absent in lung, blood vessels, skeletal muscle, and nerves.
• Post-translational modifications include hydroxylation, glycosylation, and phosphorylation, with disulfide bonds stabilizing its structure; it is also subject to alternative splicing.
• Mutations in COL17A1 are associated with junctional epidermolysis bullosa (JEB), underscoring its essential role in maintaining epithelial integrity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 1300–1400 of human COL17A1 (Q9UMD9), which lies within the extracellular region. Consider this when designing detection protocols to ensure the epitope is exposed.
• For Western blotting (WB), the predicted molecular weight is approximately 150 kDa; however, processed forms (e.g., the 120 kDa LAD-1 fragment) may also be detected depending on sample preparation and tissue type.
• In immunohistochemistry (IHC-P), validate the antibody on positive control tissues such as human skin or cornea, where strong hemidesmosomal staining is expected. Antigen retrieval methods may be required for optimal epitope exposure.
• Since COL17A1 is a transmembrane protein with secreted ectodomain fragments, consider using appropriate lysis buffers and controls to differentiate between full-length and shed forms in ELISA or other assays.
• Cross-reactivity is confirmed for human samples; performance in other species has not been tested. Validate species specificity if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a comprehensive portfolio of validated antibodies, including monoclonal and polyclonal options for hemidesmosome, basement membrane, and autoimmune blistering disease research. Whether you need bulk quantities of this anti-COL17A1 rabbit mAb, matched antibody pairs, or ancillary reagents for assay development, our team ensures efficient sourcing from concept to clinic. For targets like collagen XVII, we also support rare-target raw-material sourcing and technical consulting to accelerate your diagnostic kit development.