Anti-Collagen X/COL10A1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q03692

Core Product Specifications and Parameters

Biological Background: Collagen X/COL10A1 Function and Localization

• Collagen alpha-1(X) chain, encoded by the COL10A1 gene, is a homotrimeric component of type X collagen, a short-chain collagen specific to hypertrophic chondrocytes.
• It is a product of hypertrophic chondrocytes and localizes to the presumptive mineralization zones of hyaline cartilage, playing a critical role in endochondral ossification.
• After synthesis, it is secreted into the extracellular matrix, where it forms a network that supports cartilage matrix organization and mineralization.
• The protein contains repeat sequences typical of collagens and requires hydroxylation and calcium binding for structural stability, as indicated by UniProt keywords.
• UniProt keywords include 3D-structure, calcium, collagen, disease variant, extracellular matrix, hydroxylation, metal-binding, proteomics identification, reference proteome, repeat, secreted, and signal.
• The mature protein has a calculated molecular weight of approximately 66 kDa, as determined by amino acid sequence analysis.

Experimental Guidance and Technical Tips

• For Western blot (WB), use the recommended molecular weight of 66 kDa as a reference; consider running samples from hypertrophic chondrocyte lysates or cartilage extracts under reducing conditions. Validate with appropriate positive and negative controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval protocols (e.g., heat-induced epitope retrieval at pH 6 or 9) and validate in human or rodent bone/cartilage tissue sections where COL10A1 is expressed in the hypertrophic zone.
• For ELISA, titrate the antibody using recombinant COL10A1 protein or peptide corresponding to the immunogen (aa 521-680) to determine optimal coating and detection concentrations.
• Note that the antibody cross-reacts with human, mouse, and rat, making it suitable for translational studies across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your cartilage and bone mineralization research, we offer this Collagen X/COL10A1 rabbit polyclonal antibody alongside validated antibody pairs, recombinant proteins, and ancillary reagents to support your assay development. Let CamelBio be your sourcing partner for rare targets and bulk raw materials, accelerating your diagnostic workflows with reliable supply.