Anti-Collagen X/COL10A1 Rabbit Monoclonal Antibody for WB, ELISA - Q03692

Core Product Specifications and Parameters

Biological Background: Collagen X/COL10A1 Function and Localization

• Collagen alpha-1(X) chain (COL10A1) is a homotrimeric component of type X collagen, a short-chain collagen predominantly expressed by hypertrophic chondrocytes.
• Type X collagen localizes to the extracellular matrix of presumptive mineralization zones in hyaline cartilage, playing a structural role during endochondral ossification.
• As a secreted extracellular matrix protein, COL10A1 contains a signal peptide and undergoes post-translational modifications including hydroxylation and metal-binding (calcium).
• Mutations in COL10A1 are associated with skeletal disorders (disease variants), highlighting its critical function in cartilage development and mineralization.
• The protein has a molecular weight of ~66 kDa and features a triple-helical collagen domain characteristic of the collagen superfamily.
• Keywords: 3D-structure, Calcium, Collagen, Disease variant, Extracellular matrix, Hydroxylation, Metal-binding, Proteomics identification, Reference proteome, Repeat, Secreted, Signal.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 500-600 in the C-terminal region of human COL10A1. This region is distinct from the triple-helical domain and may confer target specificity.
• For Western blotting, anticipate a band at approximately 66 kDa. Validate the antibody in relevant cell lysates, such as chondrocyte or cartilage extracts.
• For ELISA, perform standard titration and optimization protocols. Consider using recombinant COL10A1 or cartilage-derived samples as positive controls.
• The antibody is reported to cross-react with mouse and rat COL10A1. Confirm reactivity in your specific sample systems, as cross-reactivity may be influenced by sequence homology and expression levels.
• Due to the extracellular matrix association of COL10A1, sample preparation for cartilage or mineralization zones may require enzymatic digestion or decalcification steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, high-quality monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-COL10A1 antibody aids researchers investigating cartilage biology, mineralization, and skeletal disorders. Whether you require rare target reagents or routine immunoassay components, CamelBio streamlines your procurement from concept to clinic.