Anti-Collagen VI/COL6A1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P12109

Core Product Specifications and Parameters

Biological Background: Collagen VI/COL6A1 Function and Localization

• Collagen alpha-1(VI) chain, encoded by the COL6A1 gene, is one of three alpha chains that assemble to form collagen VI, a ubiquitous extracellular matrix microfibrillar protein.
• Collagen VI functions as a cell-binding protein, mediating cell adhesion and interactions with other ECM components such as integrins, thereby influencing tissue architecture and signaling.
• Localizes to the extracellular space and is deposited into the extracellular matrix, where it contributes to the structural integrity of various connective tissues.
• Post-translational modifications include hydroxylation of proline and lysine residues and N-linked glycosylation, characteristic of collagen family members.
• Mutations in COL6A1 are associated with congenital muscular dystrophies, including Ullrich congenital muscular dystrophy (UCHMD1) and Bethlem myopathy (BTHLM1), as indicated by disease-variant annotations.
• The protein contains repeating Gly-X-Y triplets typical of collagens, forming a triple-helical domain essential for its structural role.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 37–235) corresponds to the N-terminal region of human COL6A1; this antibody is expected to recognize epitopes within this domain. Cross-reactivity with other species relies on sequence conservation in this region.
• For western blot (WB), the predicted molecular weight is ~109 kDa. Consider reducing/denaturing conditions and appropriate gel percentage for high-molecular-weight proteins. Validate specificity using positive and negative cell lysates.
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) may enhance detection. Optimize antibody dilution and include isotype controls.
• For ELISA, the antibody can be used as a capture or detection reagent. Pair with a compatible monoclonal antibody recognizing a distinct epitope to develop a sandwich assay.
• Evaluate potential cross-reactivity with other collagen VI chains (COL6A2, COL6A3) or other collagen family members, as conserved domains may lead to off-target binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, offering validated monoclonal and polyclonal antibodies, antibody pairs, bulk ancillary reagents, and technical support to streamline diagnostic development. This anti-Collagen VI/COL6A1 rabbit monoclonal antibody meets the needs of researchers and manufacturers focusing on extracellular matrix biology, cell adhesion, and inherited muscular disorders. From lead candidate identification to scale-up, CamelBio provides flexible sourcing solutions to accelerate your projects.