Anti-Collagen VI/COL6A1 Polyclonal Antibody for WB, ELISA - P12109

Core Product Specifications and Parameters

Biological Background: Collagen alpha-1(VI) chain Function and Localization

• Collagen alpha-1(VI) chain is a component of type VI collagen, a major structural element of microfibrils within the extracellular matrix.
• It functions as a cell-binding protein, mediating cell adhesion and interactions with integrins and other receptors.
• The protein is encoded by the COL6A1 gene and assembles into a heterotrimeric molecule with alpha-2(VI) and alpha-3(VI) chains to form collagen VI.
• It is secreted into the extracellular space and localized within the extracellular matrix of connective tissues, including skin, muscle, and cartilage.
• Post-translational modifications include hydroxylation and glycosylation, which are essential for proper collagen folding and stability.
• Mutations in COL6A1 are linked to congenital muscular dystrophies such as Ullrich congenital muscular dystrophy (UCHMD1) and Bethlem myopathy (BTHLM1), indicating its role in muscle function and integrity.
• The protein contains von Willebrand factor type A (vWA) domains, which facilitate protein-protein interactions critical for matrix organization.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 109 kDa; however, processed or degradation products may be observed. Optimize reducing conditions and sample preparation accordingly.
• Validate antibody performance in relevant human or mouse cell lysates or tissue extracts known to express collagen VI, such as fibroblasts or muscle tissue.
• ELISA assays can be designed to detect native or recombinant collagen VI, but capture/detection conditions should be empirically determined.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs by offering a comprehensive range of IVD raw materials, including this Collagen VI/COL6A1 antibody for extracellular matrix and muscular dystrophy studies. Beyond polyclonal antibodies, we provide validated antibody pairs, optimized monoclonal options, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinical application.