Anti-Collagen IV Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q01955 / P29400 / P08572 / P02462

Core Product Specifications and Parameters

Biological Background: Collagen IV (COL4A3 chain) Function and Localization

• Collagen alpha-3(IV) chain, also known as Goodpasture antigen, is a major structural component of type IV collagen in basement membranes.
• It forms a 'chicken-wire' meshwork together with laminins, proteoglycans, and entactin/nidogen, essential for the integrity of glomerular basement membranes (GBM).
• The NC1 domain fragment, tumstatin, exhibits anti-angiogenic and anti-tumor cell activity, potentially acting via RGD-independent ITGB3-mediated mechanisms.
• Tissue specificity: Alpha-3 and alpha-4 type IV collagens are colocalized in kidney, eye (lens capsule), cochlea, lung, skeletal muscle, aorta, and fetal tissues; isoform 1 is more abundant than isoform 3 in several tissues. Related references: PMID:8083201, PMID:7523402
• Subcellular location: Secreted, found in the extracellular space and extracellular matrix, specifically in the basement membrane.
• Post-translational modifications include hydroxylation, phosphorylation, and disulfide bond formation.
• Associated diseases include Alport syndrome and deafness, linked to mutations in the COL4A3 gene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a fragment of human COL4A3 (aa 1555-1669); consider potential cross-reactivity with other collagen IV chains and validate specificity in your system.
• For Western blotting, the predicted molecular weight is ~162 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry, expect basement membrane staining patterns; confirm with positive control tissues or cells expressing COL4A3.
• ELISA detection may be optimized using the recombinant immunogen; verify performance with native protein or complex samples.
• Reported cross-reactivity includes human and rat; when working with other species, preliminary validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with one-stop procurement of IVD raw materials, from validated antibody pairs and monoclonal antibodies to bulk ancillary reagents and rare target sourcing. This anti-Collagen IV antibody serves basement membrane biology, kidney disease, and angiogenesis research, and our team can assist with complementary raw-material needs and technical consultation for your IVD development pipeline.