Anti-Collagen III alpha 1/COL3A1 Rabbit Monoclonal Antibody for WB, IP, ELISA - P02461

Core Product Specifications and Parameters

Biological Background: Collagen alpha-1(III) chain Function and Localization

• Collagen alpha-1(III) chain, encoded by the COL3A1 gene, is a major component of type III collagen, which is predominantly found in soft connective tissues alongside type I collagen.
• It is a secreted extracellular matrix protein that undergoes extensive post-translational modifications, including hydroxylation, glycosylation, and disulfide bond formation.
• Alternative splicing generates multiple isoforms (UniProt keyword: Alternative splicing).
• In the developing brain, COL3A1 acts as the major ligand for the adhesion G protein-coupled receptor ADGRG1, inhibiting neuronal migration and activating the RhoA pathway via coupling to GNA13 and possibly GNA12.
• Mutations in COL3A1 are associated with Ehlers-Danlos syndrome, vascular type, and aortic aneurysm (UniProt keywords: Disease variant, Ehlers-Danlos syndrome, Aortic aneurysm).
• The protein binds calcium and is classified as a metal-binding glycoprotein (UniProt keywords: Calcium, Metal-binding).
• Subcellular localization is secreted, extracellular space, and extracellular matrix.

Experimental Guidance and Technical Tips

• Consider using a low-percentage SDS-PAGE gel (e.g., 6–8%) for western blotting due to the high molecular weight of COL3A1 (~139 kDa).
• Validate the antibody in the relevant species and tissue type, as collagen III abundance varies across different connective tissues.
• For immunoprecipitation, optimize the antibody concentration and incubation conditions based on your sample type and downstream application.
• In ELISA, ensure appropriate blocking and washing steps to minimize background, particularly when working with complex biological matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets like Collagen III alpha 1/COL3A1 involved in connective tissue and extracellular matrix research, CamelBio offers optimized monoclonal antibodies (such as this rabbit mAb), validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions to accelerate your assay development and rare target procurement.