Anti-Collagen III alpha 1/COL3A1 Polyclonal Antibody for WB, IF/ICC, ELISA - P02461

Core Product Specifications and Parameters

Biological Background: Collagen III alpha 1/COL3A1 Function and Localization

• Collagen alpha-1(III) chain is a major fibrillar collagen that, together with type I collagen, constitutes the structural framework of most soft connective tissues, including skin, lung, and vascular walls.
• It plays a critical role in cortical development by serving as the primary ligand for adhesion G protein-coupled receptor G1 (ADGRG1) in the developing brain; this interaction inhibits neuronal migration and activates the RhoA signaling pathway through coupling to GNA13 and possibly GNA12.
• The protein is secreted into the extracellular space and assembles into supramolecular fibrils within the extracellular matrix, where it provides tensile strength and tissue integrity.
• COL3A1 undergoes extensive post-translational modifications, including proline hydroxylation, lysine hydroxylation and glycosylation, and disulfide bond formation, which are essential for proper triple-helix stability and secretion.
• Mutations in COL3A1 are associated with Ehlers-Danlos syndrome, vascular type (EDS4A/EDSVASC) and familial thoracic aortic aneurysm, underscoring its importance in vascular wall maintenance.
• Alternative splicing generates additional transcript variants, though the functional significance is not fully characterized; the primary isoform has a calculated molecular weight of approximately 138.5 kDa.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 1201-1301) lies within the C-terminal propeptide region of human COL3A1; consider sequence alignment to assess reactivity with other collagen types or species orthologs.
• For Western blotting, the predicted molecular weight is ~139 kDa; use a 6–8% SDS-PAGE gel and extended transfer times to ensure efficient transfer of this high molecular weight protein.
• In immunofluorescence and immunocytochemistry, extracellular collagen fibrils may require permeabilization and blocking steps optimized for matrix-rich samples; validate in your specific cell or tissue model.
• For ELISA, the polyclonal nature may provide broad epitope coverage, but titration and cross-reactivity checks against other collagen family members (e.g., COL1A1) are recommended to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio streamlines access to validated antibodies, proteins, and ancillary reagents for extracellular matrix and connective tissue research. Our portfolio includes this COL3A1 rabbit polyclonal antibody alongside optimized monoclonal/polyclonal options, bulk reagents, and custom sourcing for rare targets. From concept to clinic, CamelBio supports diagnostic manufacturers and research institutions with reliable, one-stop procurement solutions tailored to your development pipeline.