Anti-Collagen II Polyclonal Antibody for WB, ELISA - P02458

Core Product Specifications and Parameters

Biological Background: Collagen II Function and Localization

• Collagen alpha-1(II) chain, encoded by the COL2A1 gene, is also known as alpha-1 type II collagen.
• Type II collagen is a major component of cartilage extracellular matrix and is specific for cartilaginous tissues.
• It plays essential roles in normal embryonic skeletal development, linear growth, and providing resistance to compressive forces in cartilage.
• The protein is secreted and localizes to the extracellular space and matrix.
• Isoform 2 shows tissue-specific expression, with high levels in juvenile chondrocytes and low levels in fetal chondrocytes.
• Key post-translational modifications include hydroxylation, glycosylation, and disulfide bond formation.
• Mutations in COL2A1 are associated with various skeletal disorders, including Stickler syndrome, dwarfism, and cataract, as well as deafness.
• Additional keywords: It is a calcium- and metal-binding protein, with a triple-helical repeat structure typical of collagens.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to amino acids 100-200 of human Collagen II. This region may be conserved across species, consistent with observed cross-reactivity to mouse and rat.
• For Western blot, consider the predicted molecular weight of ~142 kDa. Note that collagen proteins may require specific sample preparation due to their extensive post-translational modifications and triple-helical structure.
• For ELISA applications, validate the antibody with the appropriate coating buffer and detection system to ensure specificity for native or denatured Collagen II.
• As a polyclonal antibody, it may recognize multiple epitopes, which can be advantageous for detecting different isoforms or modified forms. Validate in the relevant biological matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive one-stop sourcing bridge for IVD raw materials. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies like this anti-Collagen II antibody, and bulk ancillary reagents. Whether you need rare target-specific raw materials or technical consulting from concept to clinic, CamelBio supports your entire development pipeline with reliable supply and expert guidance.