Anti-Collagen I/COL1A2 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P08123

Core Product Specifications and Parameters

Biological Background: Collagen I/COL1A2 Function and Localization

• Collagen alpha-2(I) chain, encoded by COL1A2, combines with alpha-1(I) chains to form type I collagen, a fibrillar collagen that is the most abundant collagen in vertebrates.
• Type I collagen is a member of group I collagens (fibrillar-forming collagens) and provides structural support to tissues.
• It is secreted into the extracellular space and is a major component of the extracellular matrix, particularly in tendons, ligaments, and bones.
• In bone, type I collagen fibrils are mineralized with calcium hydroxyapatite, contributing to bone strength.
• Post-translational modifications include hydroxylation of proline and lysine residues, glycosylation, and disulfide bond formation, which are critical for collagen stability.
• Mutations in COL1A2 are associated with connective tissue disorders such as osteogenesis imperfecta, Ehlers-Danlos syndrome, and certain types of dwarfism.
• Keywords indicate it is a secreted glycoprotein with metal-binding properties, and its structure has been extensively studied.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide corresponding to amino acids 501-600, the antibody may recognize linear epitopes and is well-suited for denaturing applications such as Western Blot.
• For IHC-P, consider antigen retrieval steps to expose the epitope, and validate staining patterns in known positive tissues like bone or tendon.
• In IF/ICC, optimization of fixation and permeabilization may be required to detect extracellular collagen.
• For ELISA, the antibody can be used to detect the peptide immunogen or purified collagen; validate binding to native collagen in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to high-quality IVD raw materials, including this anti-Collagen I/COL1A2 polyclonal antibody suitable for extracellular matrix and bone research. As a one-stop bridge, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, with expert support for rare target sourcing from concept to clinic.