Anti-Collagen I/COL1A2 Polyclonal Antibody for WB, IF/ICC, ELISA - P08123

Core Product Specifications and Parameters

Biological Background: Collagen alpha-2(I) Chain Function and Localization

• Collagen alpha-2(I) chain is a subunit of type I collagen, the most abundant fibrillar collagen in the human body, providing tensile strength to connective tissues.
• Together with two alpha-1(I) chains, it forms the heterotrimeric type I procollagen molecule, which undergoes proteolytic processing to assemble into mature collagen fibrils.
• Type I collagen is a major structural component of the extracellular matrix in skin, bone, tendons, ligaments, and other connective tissues, where it organizes into fibrils that are mineralized with calcium hydroxyapatite in bones.
• The protein is synthesized as a preproprotein with a signal peptide, and the mature chain is secreted into the extracellular space, contributing to the supramolecular assembly of collagen fibers.
• Tissue-specific expression: highly expressed in tendon, ligament, and bone, with fibril organization critical for biomechanical properties; mutations in COL1A2 cause osteogenesis imperfecta and Ehlers-Danlos syndrome.
• Post-translational modifications include hydroxylation of proline and lysine residues, glycosylation, and formation of disulfide bonds, which are essential for triple helix stability and fibril cross-linking.
• The protein contains a large triple-helical domain with characteristic Gly-X-Y repeats, where X is often proline and Y is often hydroxyproline, and plays a role in cell adhesion via integrin-binding sites.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 1161-1260) of human COL1A2, which may recognize both the pro-form and mature collagen; validate in your sample system.
• For Western blot, use reducing SDS-PAGE conditions to dissociate collagen trimers; note that the theoretical molecular weight of the pro-alpha-2 chain is ~129 kDa, but mature processed forms may appear smaller.
• When performing immunofluorescence (IF/ICC), consider that collagen is secreted; if staining intracellular procollagen, include permeabilization steps and co-localize with ER/Golgi markers.
• ELISA applications may be used to detect soluble collagen fragments or secreted collagen in cell culture supernatants; optimize coating and detection conditions for your matrix.
• The antibody cross-reacts with mouse and rat, supporting translational studies in animal models of fibrosis, bone disease, and wound healing.

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CamelBio empowers diagnostic and research communities with streamlined access to premium IVD raw materials. From validated antibody pairs and optimized polyclonal antibodies like this anti-Collagen I/COL1A2 pAb to bulk ancillary reagents and rare-target sourcing, we bridge your project from concept to clinic. Whether your focus is extracellular matrix biology, bone metabolism, or connective tissue disorders, our one-stop supply services help accelerate your assay development.