Anti-Collagen I/COL1A2 Monoclonal Antibody for WB, IF/ICC, ELISA - P08123

Core Product Specifications and Parameters

Biological Background: Collagen I/COL1A2 Function and Localization

• Collagen alpha-2(I) chain, also known as alpha-2 type I collagen, is encoded by the COL1A2 gene in humans.
• Type I collagen is a member of the fibrillar-forming group of collagens, functioning as a major structural component of the extracellular matrix.
• It is secreted and localized in the extracellular space, where it forms fibrils that provide tensile strength to tissues.
• The protein is highly expressed in tendon, ligaments, and bones; in bone, the fibrils are mineralized with calcium hydroxyapatite.
• Post-translational modifications include hydroxylation, glycosylation, and disulfide bond formation, which are essential for proper collagen assembly and stability.
• Mutations in COL1A2 are associated with connective tissue disorders including osteogenesis imperfecta, Ehlers-Danlos syndrome, and certain dwarfisms, underscoring its clinical significance.
• The protein contains characteristic Gly-X-Y repeats and is synthesized as a precursor with a signal peptide, followed by proteolytic processing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (aa 500-600) of human COL1A2; the antibody may detect both full-length and processed forms.
• For Western blot, expect a band around 129 kDa under reducing conditions; include collagen-rich tissue lysates (e.g., tendon, bone, skin) as positive controls.
• In immunofluorescence (IF/ICC), consider using fibroblast or osteoblast cell lines; matrix deposition may require extended culture and ascorbate treatment.
• The antibody shows cross-reactivity with mouse, enabling its use in mouse models of bone or connective tissue research.
• For ELISA, pair with a suitable capture/detection antibody and validate with recombinant collagen I or tissue extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-Collagen I/COL1A2 antibody supports research in extracellular matrix biology, connective tissue disorders, and bone mineralization—areas where reliable reagents are critical. Whether you need rare target sourcing or production-scale supply, CamelBio provides integrated solutions to move from concept to clinic efficiently.