Anti-Collagen I/COL1A2 Monoclonal Antibody for WB, ELISA - P08123

Core Product Specifications and Parameters

Biological Background: Collagen alpha-2(I) Chain Function and Localization

• Collagen alpha-2(I) chain, encoded by the COL1A2 gene, is a component of type I collagen, a fibrillar-forming collagen belonging to the group I collagen family.
• Type I collagen is the most abundant collagen in vertebrates and serves as the major structural protein of the extracellular matrix, providing tensile strength to connective tissues.
• It forms the fibrils of tendons, ligaments, and bones; in bones, these fibrils are mineralized with calcium hydroxyapatite, contributing to tissue stiffness and load-bearing capacity.
• The protein is characterized by a repetitive Gly-X-Y tripeptide motif, which facilitates stable triple-helix formation, and undergoes extensive post-translational modifications, including proline and lysine hydroxylation and disulfide bond formation.
• Mutations in COL1A2 are associated with a spectrum of connective tissue disorders, including osteogenesis imperfecta type IV (OI4) and Ehlers-Danlos syndromes (EDSCV, EDSARTH2), as well as dwarfism and chromosomal rearrangements.
• Subcellularly, collagen alpha-2(I) is synthesized as a precursor, secreted into the extracellular space, and processed to its mature form, where it assembles into supramolecular fibers.
• It is a glycoprotein with metal-binding properties, particularly for calcium, which is critical for matrix mineralization and structural integrity.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 500–600 of human COL1A2 (NP_000080.2); it is suitable for western blot (WB) and ELISA applications.
• For WB, consider using reducing and denaturing conditions; the predicted molecular weight is ~129 kDa, and a band near this size may be detected in lysates from human, mouse, or rat tissues.
• For ELISA, titrate the antibody to determine the optimal working concentration, and include appropriate positive and negative controls to ensure specificity and sensitivity in your assay system.
• Cross-reactivity is indicated for human, mouse, and rat; validate the antibody in the relevant sample type and matrix for your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-collagen I/COL1A2 antibody supports research into extracellular matrix biology, bone disorders, and connective tissue diseases. Contact us to discuss how our end-to-end supply capabilities can accelerate your diagnostic or research project.