Anti-Collagen I/COL1A1 Rabbit PolymAb® for WB - P02452

Core Product Specifications and Parameters

Biological Background: Collagen I/COL1A1 Function and Localization

• Collagen alpha-1(I) chain, encoded by the COL1A1 gene, is a key subunit of type I collagen, the most abundant fibrillar collagen in the human body.
• Type I collagen belongs to group I collagens (fibrillar-forming) and assembles into heterotypic fibrils with collagen alpha-2(I), providing tensile strength to connective tissues.
• The protein is synthesized as a precursor with N- and C-terminal propeptides, which are cleaved during maturation to enable fibril formation.
• It is a secreted extracellular matrix protein, localizing to the extracellular space of tendons, ligaments, and bones, where it supports tissue integrity.
• In bone, collagen fibrils composed of COL1A1 are mineralized with calcium hydroxyapatite, conferring rigidity.
• Mutations in COL1A1 are linked to osteogenesis imperfecta (brittle bone disease) and Ehlers-Danlos syndrome, highlighting its critical role in connective tissue health.

Experimental Guidance and Technical Tips

• For Western blot, the target molecular weight is ~139 kDa; consider using long transfer times and low-percentage gels for optimal high-molecular-weight protein resolution.
• The immunogen sequence (aa 1150-1250) lies within the triple-helical domain; thus, the antibody may recognize both pro-collagen and mature collagen forms.
• In immunohistochemistry on paraffin-embedded sections, heat-induced antigen retrieval is recommended to unmask epitopes in collagen-rich, cross-linked tissues.
• Validate cross-reactivity in mouse and rat samples by assessing sequence homology in the immunogen region, as epitope conservation may vary between species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-Collagen I/COL1A1 Rabbit PolymAb® as part of its comprehensive IVD raw-material portfolio for connective tissue and musculoskeletal disease research. As a one-stop sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and custom monoclonal antibody development to support your diagnostic assay pipeline. Contact our team for technical support and batch-specific performance data.