Anti-Collagen I/COL1A1 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P02452

Core Product Specifications and Parameters

Biological Background: Collagen I/COL1A1 Function and Localization

• Collagen alpha-1(I) chain (COL1A1) is a major component of type I collagen, the most abundant fibrillar-forming collagen in vertebrates. It belongs to the group I collagen family.
• Type I collagen is a heterotrimer consisting of two alpha-1(I) chains and one alpha-2(I) chain, forming the primary structural element of the extracellular matrix (ECM) in connective tissues.
• The protein is secreted into the extracellular space and deposited in the ECM, where it undergoes extensive post-translational modifications, including hydroxylation, glycosylation, and disulfide bond formation, to assemble into mature fibrils.
• Tissue distribution is predominantly in tendons, ligaments, and bones; in bone, the collagen fibrils are mineralized with calcium hydroxyapatite, providing tensile strength and structural integrity.
• Alternative splicing and proteolytic processing generate multiple isoforms; mutations in COL1A1 are associated with connective tissue disorders such as osteogenesis imperfecta, Ehlers-Danlos syndrome, and certain forms of dwarfism.
• Additional annotated features include metal-binding (calcium), phosphorylated residues, and a pyrrolidone carboxylic acid modification at the N-terminus, reflecting complex regulation of its maturation and function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to residues 1151–1250 of human COL1A1 (NP_000079.2), a region near the C-terminus. This design may favor detection of the full-length procollagen or mature collagen chains depending on sample processing.
• For Western blot, a band around 139 kDa is expected; reducing conditions may help resolve collagen monomers, while non-reducing conditions preserve higher-order oligomers. In tissue lysates, consider collagenase pretreatment to confirm specificity.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), collagen is predominantly extracellular; adequate permeabilization (e.g., Triton X-100) and antigen retrieval (for paraffin sections) may be required. Co-staining with ECM markers or detection in decalcified bone sections can provide additional validation.
• For ELISA applications, direct or sandwich formats can be explored. Peptide coating or capture by a second antibody may improve sensitivity when working with complex biological matrices such as serum or tissue extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research institutes with high-quality antibodies and related reagents. This collagen I/COL1A1 polyclonal antibody supports ECM and skeletal biology research; through CamelBio’s supply network, customers can also access validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for accelerated assay development and clinical translation.