Anti-Collagen I/COL1A1 Rabbit Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - P02452

Core Product Specifications and Parameters

Biological Background: Collagen I/COL1A1 Function and Localization

• Collagen alpha-1(I) chain, also known as alpha-1 type I collagen, is a component of type I collagen, which belongs to the group I collagens (fibrillar forming collagens).
• Type I collagen is the most abundant collagen and a major structural protein of the extracellular matrix, providing tensile strength to tissues.
• It is secreted into the extracellular space and extracellular matrix, where it assembles into fibrils.
• Tissue specificity: it forms the fibrils of tendon, ligaments, and bones. In bones, the fibrils are mineralized with calcium hydroxyapatite, contributing to bone strength.
• Post-translational modifications include hydroxylation, disulfide bond formation, and phosphorylation (as indicated by keywords). It is also a glycoprotein.
• Mutations in COL1A1 are associated with various connective tissue disorders, including osteogenesis imperfecta, Ehlers-Danlos syndrome, dwarfism, and osteoporosis (as indicated by UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 1151–1250). Ensure that the antibody recognizes the full-length protein in native conditions.
• For western blot, the predicted molecular weight is approximately 139 kDa. Consider using a 6–8% SDS-PAGE gel for adequate separation of high molecular weight proteins.
• This antibody cross-reacts with human, mouse, and rat, making it suitable for studies across these species. Verify reactivity in your specific sample system.
• For IHC-P and IF-P, optimize fixation and antigen retrieval protocols for the tissue type of interest, as collagen-rich tissues may require specific permeabilization steps.
• For ELISA, validate the antibody pair and assay conditions with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing this high-quality anti-Collagen I/COL1A1 rabbit monoclonal antibody as part of a specialized portfolio for extracellular matrix and bone biology research. Beyond this product, we supply validated antibody pairs, recombinant proteins, and bulk ancillary reagents, with dedicated support for sourcing rare-target raw materials to accelerate your diagnostic development from concept to clinic.