Anti-Collagen I/COL1A1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02452

Core Product Specifications and Parameters

Biological Background: Collagen I/COL1A1 Function and Localization

• Collagen alpha-1(I) chain (COL1A1) is the primary component of type I collagen, the most abundant fibrillar collagen in vertebrates, essential for structural integrity of connective tissues.
• Type I collagen is a member of group I collagen (fibrillar forming collagen), critical for providing tensile strength.
• The protein is predominantly found in the extracellular matrix of bone, tendons, and ligaments; in bone, fibrils are mineralized with calcium hydroxyapatite.
• COL1A1 is secreted and localized to the extracellular space, where it undergoes extensive post-translational processing, including hydroxylation, glycosylation, and disulfide bond formation.
• Mutations in COL1A1 are linked to connective tissue disorders such as osteogenesis imperfecta and Ehlers-Danlos syndrome, highlighting its clinical significance.
• The mature protein is approximately 138.9 kDa and contains repetitive sequences characteristic of fibrillar collagens.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 1120–1218) of human COL1A1; consider its potential to recognize both pro- and mature forms, and validate in your sample system.
• For Western blotting, expect a band near 139 kDa for full-length COL1A1; additional bands may represent processed or degradation products.
• In IHC-P and IF/ICC, optimize antigen retrieval and fixation as collagen is highly cross-linked; bone or tendon tissues may require decalcification.
• The antibody cross-reacts with mouse and rat COL1A1, making it suitable for rodent models of skeletal or connective tissue research.
• For ELISA, use appropriate controls to avoid non-specific adsorption often seen with collagen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with one-stop access to validated IVD raw materials, including polyclonal and monoclonal antibodies for collagen biology and extracellular matrix studies. Our supply capabilities encompass bulk ancillary reagents, optimized antibody pairs, and custom sourcing for rare targets like COL1A1, streamlining your workflow from concept to clinic.