Anti-Collagen alpha-1(XVI) chain (COL16A1) Rabbit Polyclonal Antibody for WB and ELISA - Q07092

Core Product Specifications and Parameters

Biological Background: COL16A1 Function and Localization

• Collagen alpha-1(XVI) chain is a member of the fibril-associated collagens with interrupted triple helices (FACIT) family, and alternative splicing yields multiple isoforms.
• It functions as a cell adhesion molecule by mediating cell attachment and inducing integrin-mediated cellular reactions, including cell spreading and morphological changes.
• The protein is secreted and localizes to the extracellular matrix, where it integrates into specialized structures such as fibrillin-1-containing microfibrils in the papillary dermis and thin, weakly banded collagen fibrils in territorial cartilage matrix.
• Tissue distribution studies reveal prominent expression in the placenta, particularly in the amnion, where it forms a dense network of reticular fibers and associates with fibroblast layers.
• COL16A1 contains collagenous domains and undergoes post-translational modifications including hydroxylation and glycosylation, essential for its structural integrity and function.
• Associated with keywords such as extracellular matrix, cell adhesion, glycoprotein, and alternative splicing, reflecting its roles in tissue organization and cellular communication.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 1000-1100) of human COL16A1; consider that the antibody may recognize full-length and some isoforms.
• For western blot, the predicted molecular weight is approximately 158 kDa; validate the detected band in your sample system and consider potential post-translational modifications that may alter migration.
• Cross-reactivity with mouse and rat has been confirmed; verify antibody specificity in these species using appropriate positive and negative controls.
• For ELISA applications, optimize coating antigen and antibody dilutions; consider pairing with a detection antibody against a different epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic developers and researchers by offering a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This COL16A1 antibody supports studies on cell adhesion and extracellular matrix biology, and our supply capabilities extend to bulk orders, custom antibody development, and rare-target sourcing—helping you accelerate from concept to clinic.