Anti-COLGALT2 Polyclonal Antibody for WB, ELISA - Q8IYK4

Core Product Specifications and Parameters

Biological Background: COLGALT2 Function and Localization

• COLGALT2 (procollagen galactosyltransferase 2) is also designated as ColGalT 2, collagen beta(1-O)galactosyltransferase 2, glycosyltransferase 25 family member 2, and hydroxylysine galactosyltransferase 2.
• It functions as a beta-galactosyltransferase that transfers beta-galactose to hydroxylysine residues of collagen, a critical modification for collagen stability and function.
• The enzyme is localized within the endoplasmic reticulum lumen, where collagen processing and glycosylation occur.
• UniProt annotates it with keywords including endoplasmic reticulum, glycoprotein, glycosyltransferase, transferase, signal peptide, and reference proteome.
• Tissue specificity data indicates expression in brain and skeletal muscle, suggesting a role in collagen-rich tissues.
• The human gene (23127), formerly known as C1orf17 and GLT25D2, encodes a protein with a predicted molecular weight of approximately 73 kDa (UniProt: 72924 Da).

Experimental Guidance and Technical Tips

• The immunogen (amino acids 28–230 of human COLGALT2) covers the N-terminal region; consider this when assessing isoform coverage and specificity.
• For western blotting, human or mouse brain or skeletal muscle lysates may be suitable positive controls, but validate in your sample system.
• Standard ELISA protocols can be applied; the polyclonal nature may offer high avidity for capture or detection setups.
• Cross-reactivity with human and mouse has been observed; for other species, perform pilot experiments.
• The predicted molecular weight is ~73 kDa; use appropriate molecular weight markers and gel percentages.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing access to validated antibodies, recombinant proteins, and ancillary reagents. Whether you need bulk quantities of this anti-COLGALT2 antibody, development of matched antibody pairs, or other collagen biology tools, our one-stop platform streamlines procurement from concept to clinic. We support diagnostic manufacturers and researchers with reliable, scalable supply chains for rare and routine targets alike.