Anti-COL6A2 Polyclonal Antibody for WB, ELISA - P12110

Core Product Specifications and Parameters

Biological Background: COL6A2 Function and Localization

• COL6A2 encodes collagen alpha-2(VI), a key component of the extracellular matrix protein type VI collagen.
• Type VI collagen forms beaded microfilaments and acts as a cell-binding protein, facilitating cell adhesion and matrix organization.
• The protein is secreted into the extracellular space and localizes to the extracellular matrix, with membrane association noted.
• Post-translational modifications such as hydroxylation, glycosylation, and phosphorylation contribute to its structural integrity and interactions.
• The gene undergoes alternative splicing, generating multiple isoforms that may have distinct functional roles.
• Mutations in COL6A2 cause collagen VI-related muscular dystrophies, including Ullrich congenital muscular dystrophy (UCMD) and Bethlem myopathy (BTHLM1).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 720-1019) of human COL6A2, which may allow detection of both full-length and truncated isoforms.
• For western blot (WB), a major band around 109 kDa is expected in human and mouse sample lysates; consider using appropriate tissue or cell line controls.
• Cross-reactivity is confirmed for human and mouse; if working with other species, validate experimentally due to sequence differences.
• For ELISA, plate coating and detection conditions may need optimization depending on the sample matrix; use standard indirect or sandwich approaches.
• As the antibody is provided in liquid form with ice bag shipping, avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For collagen VI and extracellular matrix research, we can supply high-quality antibodies like this anti-COL6A2 pAb, along with technical support to help you move from concept to clinic efficiently. Contact us to discuss your customized sourcing and supply needs.