Anti-COL5A1 Rabbit Polyclonal Antibody for WB and ELISA - P20908

Core Product Specifications and Parameters

Biological Background: Collagen alpha-1(V) (COL5A1) Function and Localization

• Collagen alpha-1(V) chain, encoded by COL5A1, is a component of type V collagen, a minor fibrillar collagen with nearly ubiquitous distribution in connective tissues. Alternative splicing generates multiple isoforms.
• Type V collagen co-assembles with type I collagen to regulate fibril diameter and organization in the extracellular matrix (ECM).
• It belongs to the group I collagen family, which includes fibrillar-forming collagens essential for tissue integrity and biomechanical properties.
• COL5A1 directly binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin, suggesting roles in cell-matrix interactions and signaling.
• The protein is secreted and localized to the extracellular space and extracellular matrix.
• Post-translational modifications include proline hydroxylation, lysine hydroxylation, sulfation, and disulfide bond formation critical for triple-helix stability.
• Mutations in COL5A1 are linked to Ehlers-Danlos syndrome (classical type), characterized by fragile skin and joint hypermobility.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the central region (aa 300-400) of human COL5A1. When using this antibody in WB, consider denaturing and reducing conditions to facilitate separation of the large ~184 kDa protein.
• For ELISA, validate the antibody with recombinant antigen or coated peptide to ensure specific binding.
• Given its recognition of human and mouse COL5A1, suitable sample lysates include fibroblasts, connective tissues, and ECM-rich preparations.
• Because collagen proteins are highly cross-linked, sample preparation may require extended homogenization and enzymatic digestion (e.g., pepsin) to achieve optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, recombinant antigens, and ancillary reagents. For collagen/ECM research, we supply optimized monoclonal and polyclonal antibodies, bulk reagents, and specialized sourcing services to support biomarker discovery and assay development. Contact CamelBio to streamline your procurement from concept to clinic.