Anti-COL4A4 Polyclonal Antibody for WB and ELISA - P53420

Core Product Specifications and Parameters

Biological Background: COL4A4 Function and Localization

• Collagen alpha-4(IV) chain, encoded by the COL4A4 gene, is a major structural component of type IV collagen, which forms a 'chicken-wire' meshwork in basement membranes together with laminins, proteoglycans and entactin/nidogen.
• Type IV collagen heterotrimers consist of alpha chains; alpha-4(IV) is typically found in combination with alpha-3(IV) and alpha-5(IV) chains, forming essential networks in specialized basement membranes.
• The protein is secreted into the extracellular matrix and is a core constituent of glomerular basement membranes (GBM), playing a critical structural role in renal filtration.
• Tissue distribution is highest in kidney, calvaria, neuroretina and cardiac muscle; lower expression in brain, lung and thymus; not detected in liver, skin, ileum, pancreas or adrenal (at protein level).
• In the eye, COL4A4 is expressed in Bruch's membrane, the inner limiting membrane, retinal layers and around blood vessels, contributing to ocular basement membrane integrity.
• Post-translational modifications include hydroxylation of proline and lysine residues, disulfide bond formation and glycosylation, essential for collagen stability and function.
• Mutations in COL4A4 are associated with Alport syndrome, a genetic disorder characterized by progressive kidney disease, hearing loss and ocular abnormalities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a specific region (aa 1573-1690) of human COL4A4; consider that the antibody may recognize a linear epitope within this domain and may not detect isoforms lacking it.
• For Western blot (WB), a major band of approximately 164 kDa is expected in human kidney or retinal tissue lysates; additional bands may reflect post-translational modifications or degradation products.
• In ELISA, optimize the coating concentration and antibody dilution using a standard indirect protocol with recombinant COL4A4 protein or tissue extracts; include appropriate negative controls.
• Validate cross-reactivity with other collagen family members if required, as polyclonal antibodies may exhibit reactivity to conserved collagenous domains.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio offers this high-quality anti-COL4A4 polyclonal antibody along with a broad portfolio of validated antibodies, recombinant proteins and ancillary reagents for basement membrane and renal disease research. Our team supports diagnostic manufacturers with one-stop access to rare-target raw materials, optimized antibody pairs and bulk supply solutions to accelerate your assay development pipeline.