Anti-COL4A3BP Polyclonal Antibody (CERT, GPBP, STARD11) - Q9Y5P4

Core Product Specifications and Parameters

Biological Background: COL4A3BP Function and Localization

• COL4A3BP is a ceramide transfer protein (CERT) also known as Goodpasture antigen-binding protein (GPBP) and START domain-containing protein 11 (STARD11). It is encoded by the CERT1 gene and has a molecular weight of ~71 kDa (UniProt: 70835 Da). Multiple isoforms may be generated via alternative splicing.
• As a key lipid transfer protein, it shelters ceramides within its steroidogenic acute regulatory lipid transfer (START) domain and mediates their non-vesicular trafficking from the endoplasmic reticulum to the Golgi apparatus for conversion to sphingomyelin. It efficiently transfers ceramides with long-chain fatty acids but not very long acyl chains. Related references: PMID:14685229, PMID:15596449, PMID:17392267
• Additionally, it can transfer diacylglycerol, although with very low efficiency. Related references: PMID:18184806
• Subcellularly, COL4A3BP localizes to the cytoplasm, Golgi apparatus, and endoplasmic reticulum, dynamically shuttling between these compartments to execute its lipid transport function.
• The protein is a phosphoprotein and lipid-binding protein, with known three-dimensional structures. It is associated with disease variants linked to intellectual disability (in the context of CERT1 mutations).
• Widely expressed across various tissues.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 369–598, covering the C-terminal START domain. This region is critical for ceramide binding, so the antibody is expected to recognize the full-length, functional protein.
• For Western blot (WB), a band at approximately 71 kDa is anticipated. Optimize antibody dilution using a relevant cell or tissue lysate expressing COL4A3BP.
• In immunofluorescence (IF/ICC), co-staining with Golgi (e.g., giantin) or ER (e.g., calnexin) markers is suggested to confirm expected subcellular localization.
• For ELISA applications, ensure that the antigen coating or detection format does not interfere with the epitope recognized by the antibody.
• Cross-reactivity with mouse and rat proteins is reported; validate in the specific sample system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive supply chain for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets like ceramide transfer protein. This COL4A3BP antibody supports research in sphingolipid metabolism, ceramide trafficking, and Golgi/ER dynamics, backed by our technical consulting from concept to clinic.