Anti-COL4A3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q01955

Core Product Specifications and Parameters

Biological Background: COL4A3 Function and Localization

• COL4A3 encodes collagen alpha-3(IV) chain, a major structural component of type IV collagen networks in basement membranes.
• It forms a 'chicken-wire' meshwork along with laminins, proteoglycans and entactin/nidogen, critical for glomerular basement membrane (GBM) integrity.
• A cleavage fragment, tumstatin (NC1 domain), possesses anti-angiogenic and anti-tumor cell activities mediated via ITGB3.
• The protein is secreted and localizes to the extracellular matrix and basement membranes, with high expression in kidney, lung, skeletal muscle, and aorta.
• Isoform 1 is predominantly expressed in kidney, lung, suprarenal capsule, muscle, and spleen.
• Mutations in COL4A3 are associated with Alport syndrome, characterized by kidney disease, hearing loss, and eye abnormalities.
• Post-translational modifications include hydroxylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 1555-1669, located in the C-terminal region of COL4A3. For WB, consider using a dilution range appropriate for the 162 kDa molecular weight under reducing conditions.
• For IF/ICC, validate the antibody in relevant cell lines or tissue sections known to express COL4A3, such as kidney or lung tissues.
• Given cross-reactivity with human, mouse, and rat, this antibody may be suitable for multiple species, but independent verification is advised for each new model system.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides comprehensive support for IVD raw-material procurement, from validated antibody pairs and bulk reagents to rare target sourcing. This COL4A3 antibody is ideal for research into basement membrane biology, kidney diseases, and anti-angiogenic mechanisms. Partner with CamelBio to streamline your diagnostic development pipeline with reliable, research-grade antibodies and ancillary materials.