Anti-COL4A2 Polyclonal Antibody for WB, ELISA - P08572

Core Product Specifications and Parameters

Biological Background: COL4A2 Function and Localization

• The COL4A2 gene encodes collagen alpha-2(IV) chain, a subunit of type IV collagen, which is the major structural component of basement membranes.
• Type IV collagen forms a 'chicken-wire' meshwork with laminins, proteoglycans, and entactin/nidogen, providing structural integrity to tissues.
• The C-terminal non-collagenous (NC1) domain, cleaved as canstatin, exhibits anti-angiogenic properties by inhibiting endothelial cell proliferation and migration and inducing apoptosis via Fas-dependent activation of procaspase-8 and -9.
• Canstatin acts as a ligand for integrins alphavbeta3 and alphavbeta5, mediating its anti-tumor effects.
• The protein is secreted and localizes to the extracellular matrix, specifically to basement membranes, as indicated by keywords like "Secreted", "Extracellular matrix", and "Basement membrane".
• Post-translational modifications include hydroxylation, bromination, disulfide bond formation, and glycosylation, which are typical of collagen proteins.
• Mutations in COL4A2 are linked to diseases such as porencephaly (POREN2) and Brain Small Vessel Disease 2 (BSVD2), highlighting its role in vascular stability.
• The full-length protein has a molecular weight of approximately 168 kDa (UniProt: 167,553 Da).

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal region (amino acids 1486–1712), which includes the NC1 canstatin domain. This suggests the antibody may recognize both full-length collagen alpha-2(IV) and the cleaved canstatin fragment.
• For Western Blot, an expected band at ~168 kDa corresponding to the full-length protein may be observed. Due to the potential detection of canstatin (~26 kDa), consider using appropriate molecular weight markers and loading controls.
• Optimize antibody dilution and incubation conditions for your specific sample type (e.g., tissue lysates, cell culture supernatants) as reactivity can vary across species and applications.
• The antibody has been reported to cross-react with human, mouse, and rat samples in WB and ELISA; validate in your experimental system to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

• As a trusted one-stop sourcing partner, CamelBio provides IVD manufacturers and researchers with high-quality raw materials, including validated polyclonal antibodies like this anti-COL4A2 antibody.
• Our catalog encompasses a broad range of antibodies, antigens, and ancillary reagents, supporting research into angiogenesis, basement membrane disorders, and related therapeutic areas.
• Leverage our streamlined supply chain to access rare-target raw materials and technical support, accelerating your diagnostic or drug discovery programs from concept to clinic.