Anti-COL4A1 Polyclonal Antibody for WB and ELISA - P02462

Core Product Specifications and Parameters

Biological Background: Collagen alpha-1(IV) chain Function and Localization

• Type IV collagen alpha-1 chain is the major structural component of glomerular basement membranes, forming a 'chicken-wire' meshwork with laminins, proteoglycans, and entactin/nidogen.
• The C-terminal NC1 domain (arresten) possesses anti-angiogenic activity, inhibiting endothelial cell proliferation, migration, and tube formation.
• Secreted protein localized to the extracellular space and basement membrane extracellular matrix.
• Highly expressed in placental tissue.
• Contains post-translational modifications including disulfide bonds, hydroxylation, and glycosylation; undergoes alternative splicing.
• Marked with the keyword 'Disease variant', indicating associations with genetic disorders affecting basement membranes.

Experimental Guidance and Technical Tips

• Given the immunogen spans aa 1445-1669 (C-terminal NC1/arresten domain), the antibody may recognize both full-length collagen IV and processed arresten fragments. Consider testing both forms in relevant samples.
• For WB, the presence of disulfide bonds could influence migration; consider comparing reducing and non-reducing conditions.
• This polyclonal antibody may be used for ELISA; validate by coating recombinant NC1 domain or using appropriate blocking to minimize background from extracellular matrix proteins.
• The protein is secreted; thus, in cell-based assays, consider analyzing conditioned media or tissue extracts rather than whole-cell lysates for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between research and diagnostic development, offering a comprehensive portfolio of IVD raw materials including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For basement membrane and angiogenesis studies, this anti-COL4A1 polyclonal antibody is available alongside technical support and consulting services to accelerate your assay development from concept to clinic.