Anti-COL2A1 Rabbit Polyclonal Antibody for WB, ELISA - P28481

Core Product Specifications and Parameters

Biological Background: COL2A1 Function and Localization

• Collagen alpha-1(II) chain (COL2A1), also known as alpha-1 type II collagen, is a major fibrillar collagen and the predominant collagen in cartilage. It is encoded by the Col2a1 gene in mice and represents the homotrimeric form of type II collagen.
• Type II collagen is essential for the normal embryonic development of the skeleton, supporting linear growth and providing the structural framework that enables cartilage to resist compressive forces, a property critical for joint function and tissue integrity.
• The protein is a member of the collagen superfamily characterized by its triple-helical domain composed of repeating Gly-X-Y motifs, where X is often proline and Y is hydroxyproline, conferring unique mechanical stability.
• COL2A1 is secreted and localized to the extracellular matrix of cartilaginous tissues, where it interacts with other matrix components such as proteoglycans and minor collagens to form a highly organized network.
• Post-translational modifications, including hydroxylation, glycosylation, and the formation of disulfide bonds, are critical for its triple-helix stability and assembly into mature collagen fibrils. The protein also binds calcium, which may influence fibril formation and matrix mineralization.
• Tissue specificity is restricted to cartilage, making COL2A1 a key marker for chondrocyte differentiation and cartilage biology research. Mutations or aberrant expression are linked to skeletal dysplasias and osteoarthritis.
• The protein undergoes alternative splicing, generating isoforms that may have distinct roles in development and disease, though the full-length form is the most abundant in healthy cartilage.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 1331-1487 of the mouse COL2A1 C-terminal region, which may confer reactivity with the native protein in mouse and rat samples; consider verifying specificity in species of interest.
• For Western blot (WB) under reducing conditions, a band in the range of 132-142 kDa (approximately 142 kDa predicted from mouse sequence) is expected in cartilage lysates; note that type II collagen can form higher-order oligomers, so optimizing sample preparation and loading controls is recommended.
• ELISA performance may be enhanced by using the same recombinant protein as a coating antigen; users should validate antibody titration and detection conditions in their specific assay format.
• As this is a polyclonal antibody, slight lot-to-lot variation may occur; it is advisable to confirm sensitivity and cross-reactivity in pilot experiments before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a broad portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and specialized tools like this anti-COL2A1 antibody for cartilage and skeletal development studies. For projects requiring custom solutions or rare-target sourcing, our team works closely with clients to secure optimized monoclonal or polyclonal antibodies and supporting reagents, streamlining the path from concept to clinic with reliable supply and technical guidance.