Anti-COL2A1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P02458

Core Product Specifications and Parameters

Biological Background: COL2A1 Function and Localization

• Collagen alpha-1(II) chain, also known as alpha-1 type II collagen, is the major structural component of cartilage extracellular matrix.
• Type II collagen is essential for normal embryonic skeletal development, linear growth, and the ability of cartilage to resist compressive forces.
• COL2A1 belongs to the collagen family and is specifically required for the formation of cartilaginous tissues.
• The protein is secreted into the extracellular space and is a key component of the extracellular matrix.
• Alternative splicing produces multiple isoforms; isoform 2 shows high expression in juvenile chondrocytes and low expression in fetal chondrocytes.
• Post-translational modifications include hydroxylation, glycosylation, and disulfide bond formation, critical for triple-helix stability.
• Mutations in COL2A1 are associated with a spectrum of disorders including Stickler syndrome, dwarfism, cataract, and deafness.
• Keywords: Extracellular matrix, collagen, glycoprotein, alternative splicing, disease variant, Stickler syndrome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1242-1487 in the C-terminal region; consider epitope location when designing detection assays.
• For Western blotting, multiple bands may be observed due to isoforms and post-translational modifications (predicted molecular weights: 30 kDa, 134 kDa, 142 kDa).
• Validate antibody performance in relevant sample types (e.g., human cartilage lysates) and consider appropriate negative controls.
• For IHC-P, antigen retrieval conditions should be optimized for cartilage tissue sections.
• ELISA applications may require pairing with a matched capture/detection antibody; evaluate cross-reactivity with other collagen types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive range of IVD raw materials and technical services—from concept to clinic. For cartilage and skeletal disease research, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents targeting COL2A1 and related collagens. Contact us to source rare-target raw materials or customize solutions for your specific assay development needs.