Anti-COL1A1 Polyclonal Antibody for WB, IF/ICC, ELISA - P02452

Core Product Specifications and Parameters

Biological Background: COL1A1 Function and Localization

• Encodes the alpha-1 chain of type I collagen, a fibrillar-forming group I collagen essential for extracellular matrix architecture.
• Major structural protein in connective tissues, forming fibrils in tendon, ligaments, and bones; mineralized with calcium hydroxyapatite in bone.
• Secreted protein localized to the extracellular space and extracellular matrix.
• Undergoes extensive post-translational modifications, including hydroxylation, glycosylation, disulfide bond formation, and phosphorylation.
• Mutations are linked to osteogenesis imperfecta, Ehlers-Danlos syndrome, osteoporosis, and certain dwarfisms.
• Binds calcium and metal ions, contributing to matrix mineralization and mechanical properties.
• Features a signal peptide, triple-helical domain, and C-terminal propeptide; possesses a pyrrolidone carboxylic acid modification.
• High abundance in bone, skin, and tendon; used as a reference for proteomics and structural studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1359–1464 within the triple-helical domain, a region conserved across species, supporting the reported human and mouse cross-reactivity.
• For Western blot, the predicted molecular weight is 139 kDa; use appropriate gel porosity (e.g., 6% SDS-PAGE) and extended transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/ICC, consider permeabilization to access intracellular procollagen pools or use without permeabilization to detect secreted extracellular matrix collagens.
• ELISA application may be suitable for quantifying soluble collagen fragments or monitoring matrix turnover; optimize coating and detection conditions.
• Due to the natural abundance of type I collagen, carefully titrate primary antibody and sample loading to avoid signal saturation or background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes with validated antibodies and bulk ancillary reagents. This COL1A1 polyclonal antibody fits extracellular matrix, connective tissue, and bone research applications. Beyond this product, CamelBio facilitates access to matched antibody pairs, optimized monoclonal/polyclonal panels, and rare-target raw-material supply, helping streamline your workflow from concept to clinic.