Anti-COL11A2 Polyclonal Antibody for WB, ELISA - P13942

Core Product Specifications and Parameters

Biological Background: COL11A2 Function and Localization

• Collagen alpha-2(XI) chain is a component of type XI collagen, a minor fibrillar collagen essential for the assembly and organization of collagen fibrils.
• It may play an important role in fibrillogenesis by controlling the lateral growth of collagen II fibrils, thereby regulating fibril diameter.
• Encoded by the COL11A2 gene, alternative splicing produces multiple isoforms that contribute to tissue-specific collagen matrices.
• The protein is secreted and localized to the extracellular matrix, where it provides structural integrity to cartilage, vitreous, and inner ear tissues.
• Characteristic Gly-X-Y repeats undergo extensive post-translational modifications, including prolyl hydroxylation and glycosylation, critical for triple helix formation and stability.
• Calcium binding and disulfide bonds further stabilize the collagen molecule; its glycoprotein nature mediates interactions with cells and other ECM components.
• Mutations in COL11A2 are linked to human disorders such as non-syndromic deafness (DFNA13, DFNB53), Stickler syndrome, and dwarfism, underscoring its role in connective tissue and auditory development.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 210–380 of human COL11A2; verify antibody specificity in your system, as cross-reactivity with other collagen family members may occur.
• For Western blotting, the predicted molecular weight is ~172 kDa, but collagen proteins often migrate at higher apparent masses due to modifications and cross-linking; use appropriate gel conditions.
• In ELISA applications, optimize coating concentration and detection parameters to account for the collagen’s tendency to self-assemble or bind nonspecifically.
• As a secreted protein, analyze conditioned medium or tissue extracts; consider that COL11A2 may require denaturing conditions for optimal solubility and epitope exposure.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare targets like collagen chains, enabling diagnostic manufacturers and researchers to advance studies in ECM biology, hearing loss, and skeletal dysplasias from concept to clinic.