Anti-COL11A1 Polyclonal Antibody for WB, IHC-P, ELISA - P12107

Core Product Specifications and Parameters

Biological Background: COL11A1 Function and Localization

• Collagen alpha-1(XI) chain is a component of type XI collagen, a minor fibrillar collagen essential for the structural integrity of cartilage extracellular matrix.
• Plays an important role in fibrillogenesis by controlling the lateral growth of collagen II fibrils, thereby regulating fibril diameter.
• Subcellularly localized to the secreted extracellular space and matrix, consistent with its role in tissue scaffolding.
• Tissue specificity: Highly expressed in cartilage, placenta, and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are predominant in cartilage, while isoforms using only exon IIB are found in tendon.
• Post-translational modifications include hydroxylation of proline and lysine residues, glycosylation, and disulfide bond formation, which are critical for triple-helix stability and supramolecular assembly.
• Contains collagenous (Gly-X-Y) repeats and non-collagenous domains, with alternative splicing generating multiple isoforms that fine-tune matrix interactions.
• Genetic variants in COL11A1 are linked to Stickler syndrome, Marshall syndrome, and non-syndromic hearing loss, underscoring its role in ocular, auditory, and skeletal development.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 260–430 within the triple-helical region, which is conserved across isoforms; the polyclonal nature may recognize both full-length and spliced variants.
• For Western blotting, consider a reducing or non-reducing gel system, as disulfide bonds in the non-collagenous domains can affect migration; the predicted molecular weight is ~181 kDa, but actual migration may differ due to post-translational modifications.
• In immunohistochemistry (IHC-P), antigen retrieval methods such as enzymatic digestion (e.g., pepsin) or heat-induced retrieval may improve detection of collagen epitopes in paraffin-embedded cartilage sections.
• For ELISA, preliminary checkerboard titrations with recombinant COL11A1 or tissue extracts are recommended to establish optimal coating and detection conditions.
• When working with rodent samples, validate cross-reactivity initially due to potential differences in isoform expression patterns between human, mouse, and rat tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies for connective tissue and skeletal disease biomarkers. This COL11A1 antibody complements our collagen-targeted offerings—from optimized monoclonal/polyclonal antibodies and bulk ancillary reagents to custom sourcing of rare targets. Whether you need consistent lot-to-lot performance for large-scale production or specialized technical consultation, CamelBio bridges your concept-to-clinic journey with reliable procurement solutions.