Anti-COL11A1 Monoclonal Antibody for WB, IF/ICC, ELISA - P12107

Core Product Specifications and Parameters

Biological Background: COL11A1 Function and Localization

• COL11A1 encodes the alpha-1 chain of type XI collagen, a minor fibrillar collagen that plays a crucial role in the assembly and maintenance of cartilage extracellular matrix by regulating the lateral growth of collagen II fibrils.
• The protein is secreted and localizes to the extracellular space and extracellular matrix (UniProt: P12107).
• It exhibits tissue-specific expression: abundant in cartilage, placenta, and some tumor or virally transformed cell lines. Alternative splicing generates isoforms with exon IIA or IIB in cartilage, while tendon expresses only the exon IIB isoform.
• COL11A1 undergoes extensive post-translational modifications, including glycosylation and hydroxylation, and contains disulfide bonds and calcium-binding sites, contributing to its structural stability.
• Mutations in COL11A1 are associated with a spectrum of connective tissue disorders, including Stickler syndrome, non-syndromic deafness (DFNA37), cataract, dwarfism, and ectodermal dysplasia.
• The protein has a predicted molecular weight of approximately 181 kDa, but multiple isoforms and processing events yield variants ranging from 167 to 182 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a specific peptide sequence (aa 301–400); confirm conservation of this epitope if extending usage to other species or isoforms.
• For Western blotting, use a low-percentage (e.g., 6–8%) SDS-PAGE gel and optimized transfer conditions for high-molecular-weight proteins; multiple bands may reflect isoform diversity.
• In immunofluorescence/immunocytochemistry, include permeabilization steps to access extracellular matrix-localized collagen, and validate with matrix-specific markers.
• For ELISA, consider using a sandwich format with a matched pair or optimize coating conditions due to the large, glycosylated nature of the antigen.
• Sample preparation for collagen extraction from tissue often benefits from pepsin or other protease treatments to release intact fibrils; note that this may alter epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering ready-to-use monoclonal antibodies like this COL11A1 Rabbit mAb, along with validated antibody pairs, ancillary reagents, and bulk supply options. Our expertise in extracellular matrix and musculoskeletal targets, combined with one-stop technical and procurement support, accelerates your diagnostic development from concept to clinic. Partner with us to efficiently secure rare-target raw materials and scale your assay.