Anti-Coilin Rabbit Monoclonal Antibody for WB, ELISA - P38432

Core Product Specifications and Parameters

Biological Background: Coilin Function and Localization

• Coilin, also known as p80-coilin, is a nuclear protein encoded by the COIL gene (GeneID: 8161). It is a key component of Cajal bodies (coiled bodies).
• It functions in the modification and assembly of nucleoplasmic small nuclear ribonucleoproteins (snRNPs), playing a critical role in pre-mRNA splicing machinery maturation.
• Coilin localizes primarily to the nucleus and is enriched in Cajal bodies, dynamic subnuclear organelles involved in RNP biogenesis.
• The protein is ubiquitously expressed across all examined cell types, reflecting its fundamental role in nuclear RNA processing.
• Coilin is subject to post-translational modifications, including phosphorylation and ubiquitin-like conjugation (isopeptide bond formation), which may regulate its activity and Cajal body dynamics.
• Structural features include repeat regions and a molecular weight of approximately 62.6 kDa (UniProt), consistent with the observed 63 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human Coilin. This region is highly conserved between human and mouse, supporting cross-reactivity.
• For western blotting, a band around 63 kDa is expected; consider optimizing sample preparation and antibody dilution for clear detection.
• This antibody is suitable for ELISA applications; validate antigen coating and detection conditions in your assay system.
• Since Coilin expression is ubiquitous, it may serve as a nuclear marker; however, confirm specificity in your cell line or tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with high-quality IVD raw materials, including validated antibodies like this anti-Coilin monoclonal antibody, which targets a key nuclear body component. We offer one-stop sourcing for antibody pairs, bulk ancillary reagents, and rare-target raw materials, streamlining your development from concept to clinic.