Anti-COG6 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y2V7

Core Product Specifications and Parameters

Biological Background: COG6 Function and Localization

• COG6 (Conserved oligomeric Golgi complex subunit 6, also known as Component of oligomeric Golgi complex 6) is a subunit of the Conserved Oligomeric Golgi (COG) complex, a multi-protein tethering complex essential for Golgi structure and function.
• COG6 is required for normal Golgi function, particularly in retrograde vesicular trafficking within the Golgi apparatus, ensuring proper glycoprotein processing and secretion.
• Localized to the Golgi apparatus membrane, COG6 acts as a membrane anchor for the COG complex, mediating tethering of COPI-coated vesicles to facilitate intra-Golgi transport.
• Mutations in the COG6 gene are associated with congenital disorder of glycosylation type IIL (CDG2L), characterized by neurological and developmental abnormalities including intellectual disability.
• Alternative splicing of COG6 generates multiple transcript variants, potentially giving rise to isoforms with distinct functional roles in protein transport.
• The protein is classified under disease variants for congenital disorder of glycosylation and intellectual disability, highlighting its critical role in cellular glycosylation pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 508-657 of human COG6, a C-terminal region that may be exposed on the cytoplasmic side of the Golgi membrane; this region is highly conserved across human, mouse, and rat.
• For Western blot (WB) analysis, consider using Golgi-enriched membrane fractions or whole-cell lysates from human, mouse, or rat cell lines; the predicted molecular weight is 73 kDa, but post-translational modifications may cause a slight shift.
• ELISA applications may benefit from using recombinant COG6 protein or peptide as coating antigen; due to the polyclonal nature, the antibody may recognize multiple epitopes and possibly different isoforms.
• Always validate the antibody in your specific sample system; cross-reactivity with mouse and rat has been indicated, but target expression levels may vary across tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to IVD raw materials like this COG6 polyclonal antibody, supporting studies on Golgi biology, protein trafficking, and congenital glycosylation disorders. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your workflow. For rare target sourcing or custom development needs, CamelBio acts as a dedicated procurement partner, ensuring efficient delivery from concept to clinic.