Anti-COG4 Rabbit Polyclonal Antibody for WB, ELISA - Q9H9E3

Core Product Specifications and Parameters

Biological Background: COG4 Function and Localization

• COG4 (Conserved oligomeric Golgi complex subunit 4) is also known as COD1, CDG2J, SWILS, and component of oligomeric Golgi complex 4.
• Functions in maintaining normal Golgi structure and function; required for SNARE-pin assembly and Golgi-to-ER retrograde transport by interacting with SCFD1. Related references: PMID:19536132 PMID:30290151
• Localizes primarily to the Golgi apparatus membrane and is also found in the cytoplasm and cytosol.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics studies.
• Classified under transport-related keywords such as 'Protein transport' and 'Retrograde transport', and is associated with congenital disorders of glycosylation (CDG2J) and dwarfism (SWILS).
• Belongs to the COG family, which forms a complex essential for tethering vesicles to the Golgi.

Experimental Guidance and Technical Tips

• The immunogen targets an internal region (aa 310–410), making the antibody suitable for detecting full-length COG4 and potentially cleavage fragments in relevant sample systems.
• For Western blot, consider using a loading control in the 80–90 kDa range due to the predicted molecular weight of 89 kDa under reducing conditions.
• Cross-reactivity with mouse and rat indicates conserved epitope(s); validate in the species-specific tissue or cell lysates to confirm reactivity.
• In ELISA, perform checkerboard titrations to optimize coating antigen concentration and antibody dilution for best signal-to-noise ratio.
• Include appropriate negative controls (e.g., pre-immune serum or antigen-blocked antibody) to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and bespoke sourcing for rare targets. This COG4 antibody supports research into Golgi biology, membrane trafficking, and congenital disorders of glycosylation, bringing reliable performance to your diagnostic or research workflows. For customized procurement or technical consultation, reach out to explore our holistic supply solutions.