Anti-COG3 Polyclonal Antibody for WB, ELISA - Q96JB2

Core Product Specifications and Parameters

Biological Background: COG3 Function and Localization

• COG3 is a subunit of the conserved oligomeric Golgi (COG) complex, also known as component of oligomeric Golgi complex 3, vesicle-docking protein SEC34 homolog, or p94.
• Plays a role in both anterograde (ER to Golgi) and retrograde (Golgi to ER) vesicular transport. Related references: PMID:11929878 PMID:37711075
• Localizes to the Golgi apparatus, specifically at the Golgi stack membrane.
• Widely expressed across tissues, with highest levels in pancreas and testis and lowest levels in lung.
• Post‑translational modifications include acetylation and phosphorylation, and the protein undergoes alternative splicing.
• Associated with congenital disorders of glycosylation, reflecting its role in glycoprotein trafficking.

Experimental Guidance and Technical Tips

• The immunogen covers the N‑terminal region (amino acids 1–300) of human COG3; therefore, the antibody may recognize epitopes in this domain. For WB, validate specificity using relevant cell lysates and consider using blocking peptides if available.
• Predicted molecular weight is 94 kDa; use appropriate gel percentages for high‑molecular‑weight protein separation and confirm band size with molecular weight markers.
• For ELISA, optimize coating antigen and antibody dilutions using checkerboard titrations. The polyclonal nature may provide signal amplification in sandwich ELISA designs.
• Cross‑reactivity with mouse and rat has been reported, enabling translational studies; however, perform species‑specific validation for your experimental system.
• Since COG3 is a peripheral membrane protein, gentle lysis conditions (e.g., non‑denaturing detergents) may improve extraction from Golgi membranes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with reliable IVD raw materials, including validated antibodies like this anti‑COG3 polyclonal antibody for Golgi transport and glycosylation studies. We offer one‑stop access to optimized antibody pairs, bulk ancillary reagents, and rare target sourcing to support your assay development from concept to clinic. Partner with CamelBio to streamline your supply chain and accelerate project timelines.