Anti-COG2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14746

Core Product Specifications and Parameters

Biological Background: COG2 Function and Localization

• COG2, also known as LDLC or CDG2Q, is a subunit of the conserved oligomeric Golgi (COG) complex, a multi-protein tethering complex essential for intra-Golgi vesicular trafficking and maintaining Golgi structure.
• The COG complex is composed of eight subunits (COG1-8) organized into two lobes; COG2 belongs to the lobe A subcomplex along with COG1, COG3, and COG4, and is critical for tethering vesicles to Golgi membranes.
• COG2 is required for normal Golgi morphology and function, ensuring proper retrograde and anterograde transport of glycosylation enzymes and thereby impacting protein glycosylation.
• Mutations in COG2 are associated with congenital disorders of glycosylation (CDG), specifically CDG type IIq (CDG2Q), characterized by defective glycosylation and severe multisystem disorders.
• The protein localizes to the Golgi apparatus membrane and is involved in membrane protein transport pathways.
• Alternative splicing of the COG2 gene generates multiple transcript variants, potentially modulating complex assembly or tissue-specific functions.
• COG2 was originally identified as a low density lipoprotein receptor defect C-complementing protein (LDLC), underscoring its role in LDL receptor stability and function.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-280) of human COG2, which is conserved across human, mouse, and rat, supporting expected cross-reactivity. Validation in the relevant sample system is recommended.
• For Western blotting, the predicted molecular weight of COG2 is approximately 83 kDa. Use appropriate loading controls and optimize blocking and detection conditions.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary due to formaldehyde fixation; consider optimizing the dilution and incubation parameters for your specific tissue type.
• When performing ELISA, the polyclonal nature of the antibody provides robust detection sensitivity; pair with suitable secondary reagents and validate linearity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering validated antibodies and ancillary reagents to accelerate diagnostic development. This anti-COG2 polyclonal antibody supports research into Golgi morphology, protein trafficking, and congenital disorders of glycosylation. Beyond this product, CamelBio provides comprehensive offerings including antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk reagents, all backed by expert technical support to streamline your path from concept to clinic.