Anti-COG1 Polyclonal Antibody for WB, IHC-P, ELISA - Q8WTW3

Core Product Specifications and Parameters

Biological Background: COG1 Function and Localization

• COG1 (Conserved oligomeric Golgi complex subunit 1) is also known as component of oligomeric Golgi complex 1, LDLB, and CDG2G. It is a subunit of the conserved oligomeric Golgi (COG) complex, a multi-protein tethering complex essential for vesicle docking and fusion at the Golgi apparatus.
• The COG1 protein is required for normal Golgi function, participating in intra-Golgi trafficking and maintaining the structural integrity of the Golgi apparatus.
• Mutations in the COG1 gene are associated with congenital disorder of glycosylation type IIg (CDG2G), highlighting its critical role in glycosylation processes within the Golgi.
• COG1 localizes to the Golgi apparatus membrane, consistent with its role in vesicle tethering and membrane trafficking.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or interactions within the COG complex.
• As a key component of the COG complex, COG1 is involved in protein transport pathways that ensure correct targeting of enzymes and other cargo within the secretory pathway.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the C-terminal region (amino acids 835-935) of human COG1. Consider this region's predicted surface accessibility when designing experiments; it may be suitable for detecting proteins in their native conformation in some applications.
• For Western blot (WB) analysis, the predicted molecular weight is ~109 kDa. Use appropriate molecular weight markers and validate the band in positive control lysates (e.g., human cell lines known to express COG1).
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and antibody dilutions using formalin-fixed paraffin-embedded tissues. Include relevant positive and negative controls to confirm specificity.
• This antibody cross-reacts with human, mouse, and rat, as indicated. When working with non-human species, verify sequence homology of the immunogen region to ensure expected reactivity.
• ELISA applications may require optimization of coating antigen and detection system. Consider using the immunogen peptide as a positive control to confirm antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, technical services, and consulting to support diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This COG1 polyclonal antibody is part of our extensive collection for Golgi biology and glycosylation disorder research. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare-target raw materials to streamline your assay development.