Anti-Cofilin Rabbit Monoclonal Antibody for WB, ELISA - P23528

Core Product Specifications and Parameters

Biological Background: Cofilin-1 Function and Localization

• Cofilin-1 (also known as CFL, 18 kDa phosphoprotein p18) is a widely expressed non-muscle isoform of the ADF/cofilin family, encoded by the CFL1 gene in humans.
• It binds to F-actin and exhibits pH-sensitive actin depolymerizing activity, crucial for regulating actin filament dynamics (PubMed:11812157, PubMed:33670794). Related references: PMID:11812157, PMID:33670794
• Together with the subcortical maternal complex, cofilin-1 is essential for zygotes to progress beyond the first embryonic cell divisions via actin regulation (PubMed:15580268). Related references: PMID:15580268
• It plays a role in epithelial cell morphology and cytoskeletal organization (PubMed:21834987). Related references: PMID:21834987
• Required for the up-regulation of the atypical chemokine receptor ACKR2 from endosomes to the cell membrane, enhancing chemokine clearance (PubMed:23633677). Related references: PMID:23633677
• Subcellularly, cofilin-1 localizes to the nucleus matrix, cytoplasm, cytoskeleton, and membrane ruffles/lamellipodia; it is also found in growth cones and axons.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from the N-terminal region (aa 1-100) of human cofilin-1; consider peptide blocking experiments to confirm specific binding.
• For western blotting, a band of approximately 19 kDa is expected; validate using appropriate positive and negative cell lysates (e.g., HeLa, Jurkat) and include loading controls.
• For ELISA applications, the antibody can function as a capture or detection reagent; consider pairing with a different anti-Cofilin clone to develop a sandwich ELISA.
• Given cofilin’s pH-sensitive depolymerizing activity, buffer pH should be carefully controlled during immunoprecipitation or immunofluorescence to maintain antibody-antigen interaction.

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