Anti-COCH Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - O43405

Core Product Specifications and Parameters

Biological Background: COCH Function and Localization

• Cochlin, also known as COCH-5B2, is encoded by the COCH gene in humans and exists as multiple isoforms due to alternative splicing.
• Functions in the regulation of cell shape and motility, particularly within the trabecular meshwork of the eye.
• Predominantly expressed in the inner ear structures, including the cochlea and vestibule, where it is secreted into the extracellular matrix.
• Subcellular localization is primarily extracellular, as a component of the extracellular matrix, following secretion.
• Contains a signal peptide, disulfide bonds, and glycosylation sites, indicating post-translational modifications important for its structure and function.
• Mutations in the COCH gene are associated with autosomal dominant non‑syndromic sensorineural deafness (DFNA9), highlighting its critical role in hearing.
• The protein features vWFA (von Willebrand factor type A) domains that mediate protein‑protein interactions within the extracellular matrix.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 20–260 of human cochlin, corresponding to the N‑terminal region following the signal peptide; this region includes the first vWFA domain and a portion of the intervening sequence.
• For IHC‑P, we recommend using formalin‑fixed, paraffin‑embedded human inner ear or trabecular meshwork tissue sections. Antigen retrieval with citrate buffer (pH 6.0) may enhance staining.
• For IF/ICC, fixation with 4% paraformaldehyde followed by permeabilization with Triton X‑100 is suggested. Titrate the antibody to determine the optimal working dilution.
• In ELISA applications, the antibody can be used as a capture or detection reagent; optimal coating concentrations and detection dilutions should be validated empirically.
• As this is a polyclonal antibody, batch‑to‑batch consistency should be confirmed for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. For projects targeting cochlin and hearing-related disorders, we offer bulk reagents, ancillary proteins, and rare‑target sourcing to streamline your assay development. From concept to clinic, CamelBio bridges the gap between discovery and diagnostic manufacturing.