Anti-Coagulation factor III/Tissue Factor Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P13726

Core Product Specifications and Parameters

Biological Background: Coagulation factor III/Tissue Factor Function and Localization

• Tissue factor (TF), also known as coagulation factor III or thromboplastin, is a transmembrane glycoprotein that serves as the primary initiator of the extrinsic blood coagulation cascade.
• TF forms a high-affinity complex with circulating factor VII (FVII) or its activated form (FVIIa); the TF:FVIIa complex proteolytically activates factor IX to IXa and factor X to Xa, leading to thrombin generation and fibrin clot formation.
• Encoded by the F3 gene, TF is expressed as a membrane-bound protein with a large extracellular domain, a single transmembrane helix, and a short cytoplasmic tail; alternative splicing may generate soluble isoforms.
• The protein is predominantly localized to the plasma membrane, but a secreted form has also been detected, consistent with its role in cell-surface assembly of coagulation complexes.
• Tissue-specific expression is highest in tissues such as lung, placenta, and pancreas, where it likely contributes to hemostatic protection and rapid clot formation upon vascular injury.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and palmitoylation, all of which contribute to proper folding, stability, and membrane anchoring.
• Keywords from UniProt highlight its involvement in blood coagulation, hemostasis, and its structural features as a membrane-anchored lipoprotein.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 120–251 of human TF, corresponding to a portion of the extracellular domain; this region is likely to recognize both full-length membrane-bound TF and any shed soluble fragments.
• For Western blotting, a band compatible with the predicted 33 kDa molecular weight may be observed; consider using reducing and non-reducing conditions to assess disulfide-linked complexes.
• In immunofluorescence/immunocytochemistry, TF is expected at the plasma membrane; appropriate permeabilization may be required for intracellular staining of nascent protein in the secretory pathway.
• The antibody shows cross-reactivity with mouse and rat TF; validation in the relevant species and tissue types (e.g., lung, placenta) is recommended to confirm detection.
• For ELISA applications, pairing as a capture or detection antibody may be explored; optimal dilutions should be determined empirically using a purified antigen or known positive sample.
• As with any polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers by supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for IVD assay development. This anti-Tissue Factor antibody, targeting a key initiator of coagulation, is available in bulk quantities to meet production needs. For customers requiring rare targets, custom immunogen design, or technical consultation from concept to clinic, CamelBio acts as a comprehensive sourcing partner.