Anti-CoA synthase (COASY) Rabbit Polyclonal Antibody for WB and ELISA - Q13057

Core Product Specifications and Parameters

Biological Background: CoA synthase (COASY) Function and Localization

• COASY is a bifunctional enzyme that catalyzes two consecutive steps in the biosynthesis of coenzyme A (CoA): the adenylation of 4'-phosphopantetheine and the phosphorylation of dephospho-CoA to CoA.
• The enzyme is known as NBP (N-terminal kinase-like) and POV-2, and belongs to the CoA synthase family.
• Alternative splicing produces multiple isoforms; isoform 2 is predominantly expressed in the brain, while the canonical isoform shows broad tissue distribution.
• COASY localizes to the cytoplasm, cytosol, and mitochondrial matrix, consistent with its role in CoA metabolism across cell compartments.
• It is expressed in all examined tissues, with highest levels in kidney and liver and lowest in peripheral blood leukocytes. Isoform 2 is mainly detected in the brain.
• COASY is a phosphoprotein and contains ATP-binding and nucleotide-binding domains, acting as both a nucleotidyltransferase and a kinase.
• Mutations in COASY are associated with neurodegenerative disorders, including neurodegeneration with brain iron accumulation (NBIA6) and pontocerebellar hypoplasia type 12 (PCH12).

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 345–564), which may detect full-length COASY and isoforms containing this segment.
• For Western blot, a band near 62 kDa is expected; consider using positive control lysates from kidney or liver and optimize gel percentage accordingly.
• In ELISA applications, standard titration of coating antigen and antibody is recommended to achieve optimal signal-to-noise ratio.
• Cross-reactivity with mouse and rat allows multispecies studies, but independent validation in each species is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like COASY—relevant to coenzyme A biosynthesis and neurodegeneration—we offer one-stop sourcing solutions, from assay development to supply of high-quality antibodies and rare target materials. Let us bridge your concept-to-clinic needs with reliable procurement and technical support.