Anti-CoA Synthase (COASY) Rabbit Monoclonal Antibody for WB and ELISA - Q13057

Core Product Specifications and Parameters

Biological Background: CoA Synthase (COASY) Function and Localization

• COASY encodes a bifunctional enzyme catalyzing the adenylation of 4'-phosphopantetheine and the phosphorylation of dephospho-CoA in the coenzyme A (CoA) biosynthetic pathway.
• The enzyme possesses both nucleotidyltransferase (PPAT) and kinase (DPCK) domains, enabling sequential ATP-dependent reactions.
• Ubiquitously expressed across tissues, with highest levels in kidney and liver; isoform 2 is predominantly found in the brain.
• Subcellular localization includes the cytoplasm and mitochondrial matrix, consistent with CoA metabolism in both compartments.
• Post-translational modifications include phosphorylation, as indicated by proteomic studies.
• Clinical mutations in COASY are associated with NBIA6 (neurodegeneration with brain iron accumulation) and PCH12 (pontocerebellar hypoplasia), highlighting its essential role in neural development and iron homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 465-564); use whole-cell lysates from human cell lines for Western blot detection of endogenous COASY (~62 kDa).
• Include a positive control such as HeLa or HEK293T lysate, and verify band specificity under reducing conditions.
• For ELISA applications, the antibody can be employed as capture or detection reagent; optimize coating and blocking conditions with recombinant COASY protein.
• Cross-reactivity is only confirmed for human; for other species, validate conservation of the immunogen sequence experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research institutes with reliable access to IVD-grade antibodies and ancillary reagents. This COASY recombinant rabbit monoclonal antibody supports studies on coenzyme A metabolism and neurodegeneration. From bulk monoclonal antibodies to custom raw-material sourcing, CamelBio streamlines your procurement with consistent quality and technical support.