Anti-CNTN4 Rabbit Polyclonal Antibody for WB and ELISA - Q8IWV2

Core Product Specifications and Parameters

Biological Background: Contactin-4 Function and Localization

• The protein is a member of the contactin family, a subgroup of the immunoglobulin superfamily, and is also known as brain-derived immunoglobulin superfamily protein 2 (BIG-2).
• It mediates cell surface interactions critical for nervous system development and exhibits neurite outgrowth-promoting activity; it may also play a role in synaptogenesis.
• Encoded by the CNTN4 gene (GeneID: 152330), alternative splicing generates multiple isoforms.
• The protein is expressed on the cell membrane as a glycoprotein; a GPI-anchored form is also secreted.
• Tissue expression is predominantly in the brain, with highest levels in the cerebellum; lower expression in other brain regions and tissues such as testis, pancreas, thyroid, uterus, small intestine, and kidney.
• Key structural features include immunoglobulin domains, disulfide bonds, and a GPI-anchor, classifying it as a lipoprotein.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 448–697 of human CNTN4, which may allow recognition of multiple isoforms if this region is conserved.
• For western blotting, a band around 113 kDa is expected for the full-length protein; however, consider that post-translational modifications or isoform variations could affect migration.
• For ELISA, the antibody can be used for antigen detection; appropriate controls and optimization are recommended for each sample type.
• As with any polyclonal antibody, validate performance in your specific experimental system, as reactivity may vary between batches.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-CNTN4 antibody supports neuroscience and cell adhesion research, and we offer sourcing for validated antibody pairs, bulk ancillary reagents, and custom requests for rare targets. Contact us to streamline your raw-material procurement and accelerate your diagnostic development.