Anti-CNTN3 Rabbit Polyclonal Antibody for WB, ELISA - Q9P232

Core Product Specifications and Parameters

Biological Background: Contactin-3 Function and Localization

• Contactin-3 is a member of the contactin family of immunoglobulin superfamily cell adhesion molecules, encoded by the CNTN3 gene (aliases: BIG-1, PANG).
• It mediates cell surface interactions during nervous system development, contributing to axonal guidance and network formation.
• Exhibits some neurite outgrowth-promoting activity, as demonstrated by similarity to related contactins.
• The protein is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) motif, placing it on the outer leaflet of the plasma membrane.
• Tissue specificity: In the brain, CNTN3 is expressed in the frontal lobe, occipital lobe, cerebellum, and amygdala, suggesting roles in both cognitive and motor pathways.
• Structurally, it contains multiple immunoglobulin-like domains and disulfide bonds, typical of adhesion molecules, and is post-translationally modified by glycosylation.
• Keywords associated with CNTN3 include: Cell adhesion, Cell membrane, Disulfide bond, Glycoprotein, GPI-anchor, Immunoglobulin domain, Lipoprotein, Membrane, Signal, Repeat.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-110 of the human CNTN3 N-terminal region; the polyclonal nature of the antibody may yield broader epitope recognition. For western blotting, a band near 113 kDa is expected.
• Given that CNTN3 is a GPI-anchored membrane protein, consider using membrane protein extraction protocols or appropriate lysis buffers to enhance detection.
• This antibody is reported to cross-react with mouse and rat CNTN3; validate specificity and sensitivity in the relevant species' tissue or cell lines before quantitative analyses.
• For ELISA applications, optimize coating concentrations and detection antibodies to match your assay format; inclusion of a recombinant CNTN3 protein control is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with a comprehensive catalog of IVD raw materials, including validated antibodies, matched antigen pairs, and bulk ancillary reagents. For researchers investigating neural cell adhesion and developmental pathways, this anti-CNTN3 antibody is part of our neural target portfolio, backed by our commitment to quality supply. Our dedicated sourcing team also supports custom procurement for rare targets and large-scale production, bridging your project from concept to clinic.