Anti-CNTN1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q12860

Core Product Specifications and Parameters

Biological Background: Contactin-1 Function and Localization

• Contactin-1 (CNTN1), also known as glycoprotein gp135 or neural cell surface protein F3, is a cell adhesion molecule belonging to the immunoglobulin superfamily.
• It plays a critical role in nervous system development by mediating cell surface interactions, particularly in the formation of paranodal axo-glial junctions in myelinated peripheral nerves.
• CNTN1 participates in oligodendrocyte generation by acting as a ligand for NOTCH1, promoting NOTCH1 activation and subsequent downstream signaling.
• Interaction with tenascin-R (TNR) induces neuronal repulsion and inhibits neurite outgrowth, contributing to axon guidance.
• The protein is strongly expressed in brain, neuroblastoma, and retinoblastoma cell lines, with lower levels in lung, pancreas, kidney, and skeletal muscle.
• Subcellularly, CNTN1 is localized to the cell membrane and is attached via a GPI-anchor; it contains immunoglobulin-like domains and disulfide bonds.
• Alternative splicing produces multiple isoforms, and post-translational modifications include glycosylation and lipidation (GPI-anchor).

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide corresponding to amino acids 250-350 of human CNTN1 (NP_778203.1). Given the polyclonal nature, it may recognize multiple epitopes and possibly various isoforms; verify specificity in your system.
• For Western blot (WB), the predicted molecular weight is ~113 kDa. Use appropriate protein standards and consider loading human, mouse, or rat brain lysates as positive controls.
• For immunohistochemistry (IHC-P), optimization of antigen retrieval (e.g., citrate buffer pH 6 or EDTA pH 8) may be required for paraffin sections; validate staining absence in negative control tissues.
• In ELISA, the antibody can be used for detection or capture; performance should be evaluated with recombinant or native CNTN1 protein.
• Ensure appropriate secondary antibody selection compatible with rabbit IgG for all applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one-stop partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing assistance for rare targets. Our supply network supports diagnostic manufacturers and researchers working on neurological targets like CNTN1 to streamline development from concept to clinic.