Anti-CNTFR Rabbit Polyclonal Antibody for WB, ELISA - P26992

Core Product Specifications and Parameters

Biological Background: CNTFR Function and Localization

• CNTFR (ciliary neurotrophic factor receptor subunit alpha) is a receptor for CNTF-related cytokines, including CNTF, CLCF1, and the CLCF1-CRLF1 heterodimer. Related references: PMID:10966616, PMID:11285233, PMID:11294841
• It functions both as a GPI-anchored membrane receptor and, after proteolytic shedding, as a soluble ligand-binding chaperone that delivers cytokine to signaling receptors. Related references: PMID:11285233, PMID:31932351, PMID:7681218
• The CNTFR complex includes the signaling subunits IL6ST/gp130 and LIFR, or IL27RA/WSX1 in the humanin receptor complex, mediating neuroprotection. Related references: PMID:10966616, PMID:11285233, PMID:11294841, PMID:19386761
• Ligand binding triggers JAK kinase activation and STAT3/STAT1 phosphorylation, driving downstream gene expression. Related references: PMID:10966616, PMID:11285233, PMID:11294841
• CNTFR is essential for motor neuron survival and oligodendrocyte progenitor proliferation, with implications in neurodegenerative diseases. Related references: PMID:11285233, PMID:19386761, PMID:31932351
• It is predominantly expressed in the nervous system and skeletal muscle; a soluble form is detected in cerebrospinal fluid and released upon peripheral nerve injury.
• Key structural features include immunoglobulin domains, disulfide bonds, and N-glycosylation, consistent with its GPI-anchored receptor role.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 90–342 of human CNTFR (extracellular region), suggesting the antibody may recognize the ligand-binding domain; consider performing epitope mapping if precise binding site is critical.
• For WB, the predicted molecular weight is approximately 41 kDa (UniProt), but glycosylation and GPI-anchor processing may cause slight shifts; validate using positive control lysates from neuronal or skeletal muscle cell lines.
• Reported cross-reactivity with mouse and rat indicates conserved epitope(s); for non-human samples, verify by sequence alignment and test in the relevant tissue or cell lysate.
• In ELISA, consider using recombinant CNTFR extracellular domain as coating antigen; optimize antibody dilution and detection conditions for your assay format.
• Always include appropriate negative controls (e.g., knockout lysates, blocking peptide) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including this anti-CNTFR polyclonal antibody for neuroscience and neuroinflammation applications. We supply validated antibody pairs, bulk ancillary reagents, and specialize in sourcing rare-target raw materials to streamline your assay development from concept to clinic. Contact us to discuss your CNTFR research needs and how we can support your project with reliable, cost-effective solutions.