Anti-CNTF Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P26441

Core Product Specifications and Parameters

Biological Background: CNTF Function and Localization

• CNTF is a cytokine that signals through the ciliary neurotrophic factor receptor (CNTFR) complex, composed of the non-signaling receptor CNTFR and the signaling subunits IL6ST/gp130 and LIFR. Related references: PMID:10966616 PMID:11285233 PMID:11294841 PMID:36930708
• Promotes neuronal survival, supports differentiation of various neuronal and non-neuronal cells, and regulates oligodendrocyte progenitor proliferation. Related references: PMID:1861138 PMID:1883844 PMID:31932351
• Ligand binding induces dimerization of IL6ST/gp130 and LIFR, activating JAK kinases (JAK1, JAK2, TYK2) that phosphorylate the receptor subunits, leading to recruitment and phosphorylation of STAT3 and STAT1. Related references: PMID:11285233 PMID:11294841
• Can also signal via trans-signaling using soluble forms of CNTFR. Related references: PMID:31932351 PMID:7681218
• Prevents the degeneration of motor axons after axotomy.
• Subcellular localization: found in the cytoplasm and is secreted.
• Keywords include developmental protein, differentiation, growth factor, and neurogenesis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 24–74 of human CNTF. For WB, the observed band may appear near the predicted 23 kDa, but consider potential post-translational modifications or cleavage that could alter migration.
• In IF/ICC or IF-P, appropriate permeabilization is recommended since CNTF is primarily cytoplasmic and secreted; validate staining in the relevant tissue/cell type.
• For ELISA, pairing with a capture antibody targeting a different epitope may enhance sensitivity; always include relevant controls.
• Cross-reactivity with mouse and rat CNTF is indicated, but sequence homology should be verified for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. This anti-CNTF polyclonal antibody supports neurobiology and cytokine signaling research, complementing our comprehensive neuroscience portfolio. Let us help you accelerate your diagnostic development from concept to clinic.